ABSTRACT
Two new 2-carboxymethyl-3-hexyl-maleic anhydride derivatives, arthrianhydride A (1) and B (2), along with three known compounds 3-5, were isolated from the fermentation broth of a grasshopper-associated fungus Arthrinium sp. NF2410. The structures of new compounds 1 and 2 were determined based on the analysis of the HR-ESI-MS and NMR spectroscopic data. Furthermore, compounds 1 and 2 were evaluated on inhibitory activity against the enzyme SHP2 and both of them showed moderate inhibitory activity against SHP2.
Subject(s)
Animals , Anhydrides/pharmacology , Biological Products/pharmacology , Enzyme Inhibitors/pharmacology , Fungi/chemistry , Grasshoppers/microbiology , Molecular Structure , Protein Tyrosine Phosphatase, Non-Receptor Type 11/antagonists & inhibitors , Secondary MetabolismABSTRACT
ABSTRACT The multi-enzyme complex (crude extract) of white rot fungi Pleurotus ostreatus, Pleurotus eryngii, Trametes versicolor, Pycnosporus sanguineus and Phanerochaete chrysosporium were characterized, evaluated in the hydrolysis of pretreated pulps of sorghum straw and compared efficiency with commercial enzyme. Most fungi complexes had better hydrolysis rates compared with purified commercial enzyme.
Subject(s)
Fungal Proteins/chemistry , Sorghum/chemistry , Cellulases/chemistry , Fungi/enzymology , Lignin/chemistry , Fungal Proteins/metabolism , Plant Stems/microbiology , Plant Stems/chemistry , Sorghum/microbiology , Cellulases/metabolism , Biocatalysis , Fungi/chemistry , Hydrolysis , Lignin/metabolismABSTRACT
Fungal metabolites are promising alternatives for the development of biorational pesticides. In this sense, microfungi from tropical regions are valuable sources of natural compounds for pest management. With the aim of broadening the search for new eco-friendly products to manage plant pests, this study was carried out to evaluate the biological activity of 23 tropical fungal extracts on three species of phytophagous insects and a plant parasitic nematode. In addition, the active principles of the most effective extract were identified. The insect deterrent activity of fungal extracts was evaluated on the settling of aphids Myzus persicae and Rhopalosiphum padi, and on the feeding of lepidoptera larva Spodoptera littoralis; the nematostatic activity was evaluated on the mobility of Meloidogyne javanica. Active metabolites from Gliomastix masseei were identified by GC-MS techniques and by comparison with commercial standards. Results showed seven extracts with strong effect on the settling of M. persicae and R. padi (settling inhibition >80%). The calculated median of effective concentration (EC50) values ranged from 8 to 38 µg/cm² for the extracts of Clonostachys rosea and G. masseei, respectively. Bioassay-guided separation of the ethyl acetate extract of G. masseei revealed the presence of fatty acids and their derivatives, where methyl 9-octadecenoate was the most active compound with EC50 values of 16 µg and 35 µg/cm² for M. persicae and R. padi, respectively. Extracts of C. rosea and G. masseei could be a promising option in the control of pest aphids in agriculture.
Los metabolitos fúngicos son agentes prometedores para el desarrollo de plaguicidas biorracionales. En este sentido, los hongos microscópicos de zonas tropicales representan una valiosa fuente de compuestos naturales para el manejo de plagas. Con la finalidad de ampliar la investigación en productos amigables con el medio ambiente, en este estudio se evaluó la actividad biológica de 23 extractos de hongos sobre 3 especies de insectos fitófagos y un nematodo fitoparásito; además se identificaron los componentes del extracto más activo. El efecto disuasivo de los extractos fúngicos se evaluó en el asentamiento de Myzus persicae y Rhopalosiphum padi, así como en la alimentación de Spodoptera littoralis; la actividad nematostática se evaluó sobre la movilidad de Meloidogyne javanica. Los metabolitos activos de Gliomastix masseei se identificaron por cromatografía de gases-espectrometría de masas y por comparación con muestras comerciales. Los resultados mostraron 7 extractos con fuerte efecto en la inhibición del asentamiento (> 80%) de M. persicae y R. padi. Los valores de la concentración efectiva media (CE50) estuvieron en el rango de 8 a 38 µg/cm² para los extractos de Clonostachys rosea y G. masseei, respectivamente. El extracto de acetato de etilo de G. masseei se fraccionó por un proceso biodirigido y reveló la presencia de ácidos grasos y sus derivados, donde el 9-octadecenoato de metilo fue el más activo, con una CE50 de 16 µg/cm² para M. persicae y 35 µg/cm² para R. padi. Los extractos fúngicos de C. rosea y G. masseei pueden ser una alternativa promisoria en el control de áfidos que son plaga en la agricultura.
Subject(s)
Animals , Biological Control Agents , Fungi , Insecta , Aphids , Plants , Fungi/chemistry , Larva , MexicoABSTRACT
Abstract We evaluated the antimicrobial activity of Aspilia latissima - an abundant plant from the Brazilian Pantanal region - against Candida albicans, Candida parapsilosis, Candida krusei, Candida tropicalis, Pseudomonas aeruginosa, Enterococcus faecalis, Escherichia coli and Staphylococcus aureus. The crude extracts and fractions showed activity in all tested microorganisms. The chloroform fraction of the leaves and roots showed the most antimicrobial activity against S. aureus, with an MIC of 500 μg/mL. This fraction was submitted to bioautographic assays to characterize the activity of the compounds. Two bands from the leaves (L-A and L-B) and three bands from the roots (R-C, R-D and R-E) were bioactive. Within the root-derived bands, the terpene derivatives stigmasterol, kaurenoic acid and kaura-9(11), 16-dien-18-oic acid were identified. Antibiotic activity of A. latissima is reported for the first time.
Subject(s)
Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/drug effects , Anti-Bacterial Agents/pharmacology , Antifungal Agents/chemistry , Antifungal Agents/drug effects , Antifungal Agents/pharmacology , Asteraceae/chemistry , Asteraceae/drug effects , Asteraceae/pharmacology , Bacteria/chemistry , Bacteria/drug effects , Bacteria/pharmacology , Brazil/chemistry , Brazil/drug effects , Brazil/pharmacology , Fungi/chemistry , Fungi/drug effects , Fungi/pharmacology , Gas Chromatography-Mass Spectrometry/chemistry , Gas Chromatography-Mass Spectrometry/drug effects , Gas Chromatography-Mass Spectrometry/pharmacology , Microbial Sensitivity Tests/chemistry , Microbial Sensitivity Tests/drug effects , Microbial Sensitivity Tests/pharmacology , Plant Extracts/chemistry , Plant Extracts/drug effects , Plant Extracts/pharmacology , Plant Leaves/chemistry , Plant Leaves/drug effects , Plant Leaves/pharmacology , Plant Roots/chemistry , Plant Roots/drug effects , Plant Roots/pharmacologyABSTRACT
Se evaluó la presencia de hongos endófitos en hojas de dos plantas medicinales, Acanthospermum australe y Pterocaulon alopecuroides, utilizadas entre otras cosas para tratar infecciones cutáneas. El muestreo se realizó en el entorno rural de dos ciudades del nordeste argentino. Los especimenes fueron recogidos por aborígenes de la zona conocedores de su distribución ambiental. Los hongos se aislaron mediante la técnica de Bisseger et al., 1994 y se identificaron empleando caracteristicas morfofisiológicas. Considerando las dos plantas, se encontraron 40 morfoespecies, donde los taxa más frecuentes en Acanthospermum australe fueron: Alternaria alternata, Aureobasidium pullulans, Fusarium oxysporum, F.solani, Myrothecium roridum, Phoma spp. y Sordaria fimícola, y en Pterocaulon alopecuroides fueron: Alternaria alternata, Aureobasidium pullulans, Cladosporium cladosporioides, Curvularia lunata, Fusarium proliferatum y Myrothecium roridum. Estos taxas considerados como generalistas (o de múltiples hospedadores), presentan según la literatura específica analizada, alta producción de metabolitos secundarios bioactivos con potenciales capacidades terapéuticas antimicrobianas.
The presence of endophytic fungi in leaves of two medicinal plants (Acanthospermum australe andPterocaulon alopecuroides) used mainly to treat skin infections, was evaluated. The sampling was performed in the rural area of two cities of northeast Argentina.The specimens were collected by the aboriginals who know the ®medicines¼. Fungi were isolated employing the Bisseger et al., 1994 technique and they were identifying by morpho fisiological characteristics. Regarding the two plant species, 40 morphospecies were found. The most frequent taxa in Acanthospermum australe were: Alternaria alternata, Aureobasidium pullulans, Fusarium oxysporum, F. solani, Myro-thecium roridum, Phoma sp. and Sordaria fimícola, and inPterocaulon alopecuroides were: Alternaria alternata, Aureobasidium pullulans, Cladosporium cladosporioides, Curvularia lunata, Fusarium proliferatum y Myrothecium roridum. These species considered generalistics (found in multiple hosts), presented in the analized literature a high production of bioactive secondary metabolites with eventual antimicrobial therapeutic properties.
Subject(s)
Fungi/isolation & purification , Fungi/classification , Fungi/growth & development , Fungi/physiology , Fungi/chemistry , Plants, Medicinal/microbiology , Skin Diseases, Infectious , ArgentinaABSTRACT
Recently, glycosphingolipids have been attracting attention due to their role on biological systems as second messengers or modulators of signal transduction, affecting several events, which range from apoptosis to regulation of the cell cycle. In pathogenic fungi, glycolipids are expressed in two classes: neutral monohexosylceramides (glucosyl-or galactosylceramide) and acidic glycosylinositol phosphorylceramides (the latter class carries longer glycan chains). It is worth to mention that monohexosylceramides exhibit significant structural differences in their lipid moieties compared to their mammalian counterparts, whereas the glycosylinositol phosphorylceramides exhibit remarkable structural differences in their carbohydrate moieties in comparison to mammal glycosphingolipids counterpart. We observed that glycosylinositol phosphorylceramides are capable of promoting immune response in infected humans. In addition, inhibiting fungal glycosphingolipid biosynthetic pathways leads to an inhibition of colony formation, spore germination, cell cycle, dimorphism and hyphal growth. Other pathogens, such as trypanosomatids, also present unique glycolipids, which may have an important role for the parasite development and/or disease establishment. Regarding host-pathogen interaction, cell membrane rafts, which are enriched in sphingolipids and sterols, participate in parasite/fungal infection. In this review, it is discussed the different biological roles of (glyco) (sphingo) lipids of pathogenic/opportunistic fungi and trypanosomatids.
Recentemente, glicoesfingolipídeos têm atraído atenção devido ao seu papel na biologia celular como segundo-mensageiro ou moduladores da transdução de sinal, afetando vários eventos, desde apoptose até a regulação do ciclo celular. Em fungos patogênicos, existem duas classes de glicolipídeos: monohexosil ceramidas neutras (glucosil-ou galactosilceramida) e glicosilinositol fosforilceramidas (os quais apresentam cadeias de carboidratos mais longas). É importante enfatizar que as monohexosil ceramidas exibem diferenças estruturais nas suas porções lipídicas quando comparadas às de mamíferos, enquanto que glicosilinositol fosforilceramidas exibem diferenças estruturais marcantes em suas porções carboidratos em comparação aos glicoesfingolipídeos de mamíferos. Observamos também que glicosilinositol fosforilceramidas são capazes de promover resposta imune em indíviduos infectados. Além do mais, inibição das vias biossintéticas de glicoesfingolipídeos de fungos acarreta a inibição da formação de colônias, germinação de esporos, ciclo celular, dimorfismo e crescimento de hifas. Outros patógenos, como os tripanosomatídeos, também apresentam glicolipídeos únicos, os quais apresentam um papel importante para o desenvolvimento do parasita e/ou para o estabelecimento da doença. Em relação à interação hospedeiro-patógeno, os "membrane rafts", estruturas da membrana plasmática enriquecidas em esfingolipídeos e esteróis, têm participação fundamental na infecção do parasita/fungo. Nesta revisão, discutimos os diferentes papéis biológicos dos (glico) (esfingo) lipídeos de fungos patogênicos/oportunistas e de tripanosomatídeos.
Subject(s)
Animals , Humans , Fungi/chemistry , Glycolipids/physiology , Leishmania/chemistry , Sphingolipids/physiology , Fungi/physiology , Glycosylphosphatidylinositols/physiology , Host-Pathogen Interactions/physiology , Leishmania/physiology , Membrane Proteins/physiologyABSTRACT
The macrofungi specimens were collected and identified from locations around Batman province of Turkey. A group of 21 macrofungi were collected from 29 different locations and analysed for the heavy metal contents. The highest Pb and Cu levels were found in Russula rubroalba while the highest Cd level was determined for the species Agaricus xanthodermus. Fe level was maximum for the species Funalia trogii; Mn for Mycena personsii; Zn level was for Morchella esculenta and Co level was for the species Agaricus xanthodermus. The lowest Cd, Cu, Mn and Zn contents were found in Inotus hispidus.
Subject(s)
Agaricales/chemistry , Animals , Cadmium/analysis , Copper/analysis , Environmental Monitoring , Fungi/chemistry , Geologic Sediments/analysis , Lead/analysis , Manganese/analysis , Metals, Heavy/analysis , Rats , Turkey , Water Pollutants, Chemical/analysis , Zinc/analysisABSTRACT
Siderophores of twenty fungi belonging to Zygomycotina (5 Mucorales), Ascomycotina (7 aspergilli, 6 penicillia, Neurospora crassa) and Deuteromycotina (Fusarium dimerum) were examined for their chemical nature. Siderophores produced by fungi other than Mucorales were all hydroxamates. Mucorales produced carboxylate siderophores. Catecholate type of siderophores were not detectable. Hydroxamate siderophores were mostly (9 out of 15) trihydroxamates, while six were dihydroxamates. Monohydroxamate nature was not shown by any of the 15 test fungal siderophores. In ligand properties, 12 out of 15 hydroxamate siderophores formed hexadentate ligands, while two formed tetradentates and one bidentate. There was good correlation between number of hydroxamate groups and ligand property.
Subject(s)
Aspergillus/chemistry , Carboxylic Acids/chemistry , Fungi/chemistry , Fusarium/chemistry , Hydroxamic Acids/chemistry , Mucorales/chemistry , Neurospora crassa/chemistry , Penicillium/chemistry , Siderophores/chemistry , Species SpecificityABSTRACT
Dimorphism in pathogenic fungi is reviewed. The phenomenon is divided into four interwoven events: (a) perception of external stimuli by cellular sensors; (b) translation into a biochemical message; (c) alteration of the genomic expression, and (d) structural reorganization towards the morphological change. Experimental evidence is provided. Finally, the possibility that fungal dimorphism may have arisen multiple times throughout evolution, is discussed
Subject(s)
Fungi/genetics , Paracoccidioides/genetics , Cell Wall/chemistry , Fungi/chemistryABSTRACT
Estudio llevado a cabo en el Centro de Enseñanza e Investigación Agropecuaria de Chiriquí de la Facultad de Agronomía, durante los meses de julio a noviembre de 1985. El diseño experimental fue el de bloques completamente randomizados con 11 tratamientos y 4 repeticiones. Los fungicidas utilizados fueron: